《植物生理学报》 2012, 48(4): 369-374

硒在苹果叶片中的赋存形式

宁婵娟^1,2,3, 朱超^1,3, 王孟熙⁴, 冀爱青^2,6, 杨燕君^1,3, 刘晓华^1,3, 张燕⁵, 吴国良^1,3,*

1河南农业大学园艺学院, 郑州450002; 2山西农业大学园艺学院, 山西太谷030801; 3河南省果树瓜类生物学重点实验室, 郑州450002; 4邓庄镇人民政府, 山西襄汾041599; 5信阳农业高等专科学校, 河南信阳464000; 6晋中学院生命科学与技术学院;山西榆次030600

通信作者：5, 吴国；E-mail: wugood2002@yahoo.com.cn；Tel: 0371-63558798

摘　要：

通过筛选‘红富士’苹果适宜的叶面施硒浓度, 研究了营养元素硒在苹果叶片中的赋存形式、分布及含硒大分子的提取分离技术。结果表明, (1) ‘红富士’苹果最佳叶面施硒浓度为100 mg·L-1, 此处理的叶片总硒含量为2.7283 mg·kg-1 (FW), 其中有机硒含量为2.3384 mg·kg-1 (FW), 有机化程度达85.71%。(2)无论在对照组还是处理组, 叶片中的有机硒均主要以蛋白质结合态存在, 平均占有机硒的77.09%。(3)蛋白质、多糖和核酸3种有机物在100 mg·L-1最优处理组的叶片中结合硒的量分别占有机硒含量的77.42%、18.46%和0.54%。(4)在100 mg·L-1处理组的蛋白质组分中, 又以盐溶性蛋白质结合硒的量最多, 占蛋白质结合态硒含量的46.27%。

关键词：硒; 赋存形式; 苹果叶片

收稿：2011-12-12   修定：2012-02-14

资助：国家自然科学基金(31171943)和河南省重大科技攻关项目(92101110600)。

Combined Forms of Selenium in Apple Leaves

NING Chan-Juan1,2,3, ZHU Chao1,3,WANG Meng-Xi4, JI Ai-Qing2,6, YANG Yan-Jun1,3, LIU Xiao-Hua1,3, ZHANG Yan5, WU GuoLiang1,3,*

1College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China; 2College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi 030801, China; 3Henan Key Laboratory of Fruit and Cucurbit Biology, Zhengzhou 450002, China; 4Government of Dengzhuang Town, Xiangfen, Shanxi 041599, China; 5Xinyang Agricultural College, Xinyang, Henan 464000, China;6Faculty of Biology Science and Technology, Jinzhong University, Yuci, Shanxi 030600, China

Corresponding author: ; E-mail: wugood2002@yahoo.com.cn; Tel: 0371-63558798

Abstract:

Combined forms, distribution of selenium in apple leaves, extraction and separation technologies of macromolecule containing selenium were studied through the selecting of best concentration of selenium for foliar application on ‘Red Fuji’ apple. The results showed that: (1) Selenium of 100 mg·L^-1was the optimal choice for ‘Red Fuji’ apple trees. Treated with this concentration, the total selenium content in apple leaves was 2.7283 mg·kg^-1 (FW), including organic selenium 2.3384 mg·kg^-1 (FW). Organic degree was 85.71%. (2) Organic selenium mainly existed as protein combined form, which accounted for 77.09% of total organic selenium, both in control group and treatment groups. (3) The combined percentages of selenium with protein, polysaccharide and nucleic acid were 77.42%, 18.46% and 0.54% respectively in apple leaves of optimum treatment 100 mg·L^-1. Therefore, Se protein was the main combined form of selenium in apple leaves. (4) Among the protein components, salt-solubility protein combined the most selenium, accounting for protein selenium content 46.27% in optimum treatment 100 mg·L^-1.

Key words: selenium; combined forms; apple leaves